Kathryn Ayscough Lab: Competitive Binding of Actin and SH3 domains at Proline-rich regions of Las17/WASP Regulates Actin Polymerisation
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239367 | PKA1284 | GST-Las17 (342-394) minimal component |
| 239368 | PKA1247 | S.cerevisiae Sla1-SH3#1-2 (5-131) in pGEX6P1 |
| 239369 | PKA1248 | S.cerevisiae Sla1-SH3#1-3 (5-413) in pGEX6P1 |
| 239370 | PKA1280 | His-Las17 (300-422) pTOPO 6xHis-Las17 (300-422) as a BamH1-HindIII fragment |
| 239371 | PKA1317 | S.cerevisiae Sla1-SH3#1-2 (5-131) W108A (mutation preventing binding to 2nd SH3) |
| 239372 | PKA1336 | GST-Las17 (300-422) RR(319,322)AA |
| 239373 | PKA1252 | GST-Sec4 (full length) cloned in BamH1 and Sal1 sites of pGEX-6P1 |
| 239375 | PKA1279 | GST-Las17 (300-422) P388A |
| 239376 | PKA1337 | GST-Las17 (300-422) RR(319,322)AA, RR(349,350)AA, and RR(382,383)AA |