A split ribozyme system for in vivo plant RNA imaging and genetic engineering.

Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 10.1111/pbi.14612. Epub 2025 Feb 7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
239387	pXYB033	Super folding Green Fluorescence Protein (sfGFP)
239574	pXYB034	Ribozyme split sfGFP expression
239575	pXYB035	Deactivated ribozyme split sfGFP expression
239576	pXYB028	Ribozyme split GFPuv expression
239577	pXYB031	Deactivated ribozyme split GFPuv expression
239578	pXYB082	Plant RNA Vision constructs with 41 bp gRNAs together with RFP input
239579	pXYB083	Plant RNA Vision constructs with 82 bp gRNAs together with RFP input
239628	pXYB089	Plant RNA Vision constructs with no gRNAs together with RFP input
239629	pXYB147	Plant RNA Vision constructs with with 82 bp gRNAs targeting AT3G20840
239630	pXYB151	Expression vector of AT3G20840
239631	pXYB155	Plant RNA Vision constructs with with 82 bp gRNAs targeting Tobacco rattle virus (TRV)
239632	pXYB156	Plant RNA Vision constructs with with 82 bp gRNAs targeting Tobacco rattle virus (TRV)
239633	pXYB162	Ribozyme split sfGFP linked with GFPuv via T2A expression
239634	pXYB163	Deactivated ribozyme split sfGFP linked with GFPuv via T2A expression
239635	pXYB164	sfGFP linked with GFPuv via T2A expression
239636	pXYB084	Plant RNA Vision constructs with 123 bp gRNAs together with RFP input
239637	pXYB085	Plant RNA Vision constructs with 164 bp gRNAs together with RFP input
239638	pXYB086	Plant RNA Vision constructs with 325 bp gRNAs together with RFP input
239639	pXYB105	Expression vector of GFPuv with 3’ tEvopreq motif
239640	pXYB106	Expression vector of Ribozyme split GFPuv with tEvopreq motif
239641	pXYB107	Expression vector of deactivated ribozyme split GFPuv tEvopreq motif
239644	pXYB108	Expression vector of GFPuv with 3’ gq2 motif
239645	pXYB109	Expression of Ribozyme split GFPuv with gq2 motif
239647	pXYB111	Expression vector of GFPuv with 3’ Triplex motif
239648	pXYB112	Expression vector of Ribozyme split GFPuv with Triplex motif
239649	pXYB113	Expression vector of deactivated ribozyme split GFPuv with Triplex motif
239650	pXYB176	Expression vector of ribozyme with IGS2 split GFPuv
239651	pXYB177	Expression vector of deactivated ribozyme with IGS2 split GFPuv