Alexis Komor Lab: Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239448 | pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening)) | Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing. |
| 239450 | pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP | Codon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker. |
| 239461 | pSG1016-2xBsaI-SaKKH_P2A_EGFP | Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker. |
| 239462 | pSG1039-rA1-SaKKH_P2A_EGFP | Cytosine base editor with codon-optimized S. aureus KKH Cas9 and rAPOBEC1 deaminase. EGFP can be used as a transfection marker. |
| 239463 | pSG1040-rA1-SaKKH-2xUGI_P2A_EGFP | Cytosine base editor with codon-optimized S. aureus KKH Cas9, rAPOBEC1 deaminase, and 2x UGI. EGFP can be used as a transfection marker. |