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Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis.

He L, Yao Y, You Y, Wei X, Ma Y, Yuan W, Lang Z, Zhu JK
Nat Commun. 2025 Dec 6. doi: 10.1038/s41467-025-66933-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
239470pMD18T-AtU6-HV5Encodes a gRNA expression cassette driven by the AtU6 promoter.
239477pMD18T-AtU3b-HV5Encodes a gRNA expression cassette driven by the AtU3b promoter.
239478pMD18T-At7SL-HV5Encodes a gRNA expression cassette driven by the At7SL promoter.
239479p1300-dCas9-HV5An intermediate vector is used to assemble three gRNAs via Golden Gate cloning.
239480dCas9-KYP (M6)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.
239482MQ1v-dCas9 (M14)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.
239483MQ1v-dCas9-KRAB (M22)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.
239484MQ1v-dCas9-SRDX (M30)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.
239485MQ1v-dCas9-BFP (M39)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.
239486MQ1v-dCas9-cSRDX (M40)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.
239489TET1cd-dCas9-p300 (D18)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.
239490TET1cd-dCas9-IBM1cd (D24)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.
239492TET1cd-dCas9-IBM1 (D30)Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Antibodies from Article