Joel Blanchard Lab: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout
Goldman, C., Kareva, T., Sarrafha, L., Schuldt, B.R., Sahasrabudhe, A., Ahfeldt, T., and Blanchard, J.W
iScience 28 (2025)
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239552 | pFUW H2B-mTagBFP2-P2A-PuroR | Expression and nuclear localization of mTagBFP2 |
| 239553 | pFUW H2B-mEmerald-P2A-PuroR | Expression and nuclear localization of mEmerald |
| 239554 | pFUW H2B-mCherry-P2A-PuroR | Expression and nuclear localization of mCherry |
| 239555 | pFUW LAMP1Sig-mEmerald | Expression and lysosomal localization of mEmerald |
| 239556 | pFUW LAMP1Sig-mCherry | Expression and lysosomal localization of mCherry |
| 239557 | pFUW COX8ASig-mEmerald | Expression and mitochondrial localization of mEmerald |
| 239558 | pFUW COX8ASig-mCherry | Expression and mitochondrial localization of mCherry |
| 239559 | pFUW PuroR-P2A-COX8ASig-Timer | Expression and mitohcondrial localization of Timer |
| 239560 | pFUW COX8ASig-Lemon | Expression and mitochondrial localization of Lemon, a pH biosensor |
| 239561 | pFUW mito-GRX1-roGFP2 | Expression and mitochondrial localization of Grx1-roGFP2 |
| 239562 | pFUW mTagBFP2 | Cytosolic expression of mTagBFP2 |
| 239563 | pFUW mEmerald | Cytosolic expression of mEmerald |
| 239564 | pFUW mCherry | Cytosolic expression of mCherry |
| 239565 | pFUW mTagBFP2-P2A-PuroR | Cytosolic expression of mTagBFP2 |
| 239566 | pFUW mEmerald-P2A-PuroR | Cytosolic expression of mEmerald |
| 239567 | pFUW mCherry-P2A-PuroR | Cytosolic expression of mCherry |
| 239568 | pFUW mTagBFP2-P2A-NeoR | Cytosolic expression of mTagBFP2 |
| 239569 | pFUW mEmerald-P2A-NeoR | Cytosolic expression of mEmerald |
| 239570 | pFUW mCherry-P2A-NeoR | Cytosolic expression of mCherry |
| 239571 | pFUW PRKN-P2A-PuroR | Overexpression of human PARK2 |