Cost-effective production of Escherichia coli "GABase" for spectrophotometric determination of gamma-aminobutyrate (GABA) levels or glutamate decarboxylase activity.
Benidickson KH, Symonds KF, Snedden WA, Plaxton WC
Biol Methods Protoc. 2025 Jun 20;10(1):bpaf050. doi: 10.1093/biomethods/bpaf050. eCollection 2025.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239572 | pET30a-GABAT | Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography. |
| 239573 | pET30a-SSDH | Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography. |