Cost-effective production of Escherichia coli ‘GABase’ for spectrophotometric determination of γ-aminobutyrate (GABA) levels or glutamate decarboxylase activity
Benidickson KH, Symonds KF, Snedden WA, Plaxton WC
Biology Methods and Protocols, 2025. doi: 10.1093/biomethods/bpaf050
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239572 | pET30a-GABAT | Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography. |
| 239573 | pET30a-SSDH | Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography. |