In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.

McDonald JL, Shapiro NP, Mengiste AA, Kaines S, Whitney SM, Wilson RH, Shoulders MD
Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2505083122. doi: 10.1073/pnas.2505083122. Epub 2025 Jun 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
239580	pACYC-prk:nptII	Selection plasmid for Rubisco directed evolution
239581	BAC-GWS1B	Single copy expression vector for GWS1B Rubisco
239582	pTrc-GWS1B	High copy vector for expression of GWS1B Rubisco