High-resolution profiling reveals coupled transcriptional and translational regulation of transgenes.
Peterman EL, Ploessl DS, Love KS, Sanabria V, Daniels RF, Johnstone CP, Godavarti DR, Kabaria SR, Oakes CG, Pai AA, Galloway KE
Nucleic Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239686 | pKG2039_pPV1-CAG | BsaI Golden Gate assembly plasmid encoding the CAG promoter |
| 239687 | pKG2019_pPV1-EF1a | BsaI Golden Gate assembly plasmid encoding the EF1a promoter |
| 239689 | pKG1988_pPV1-UbC | BsaI Golden Gate assemby plasmid encoding the UbC promoter |
| 239691 | pKG1367_pPV1-hPGK | BsaI Golden Gate assembly plasmid encoding the hPGK promoter |
| 239693 | pKG0743_pPV1-ZF43x6-C | BsaI Golden Gate assembly plasmid encoding the COMET ZF43x6-C promoter |
| 239694 | pKG3657_pPV1-ZF10.BSx8 | BsaI Golden Gate assembly plasmid encoding the synZiFTR ZF10.BSx8 promoter |
| 239695 | pKG0586_pPV2-mRuby2 | BsaI Golden Gate assembly plasmid encoding the mRuby2 CDS |
| 239696 | pKG2387_pPV2-mRuby2-P2A-PuroR | BsaI Golden Gate assembly plasmid encoding the mRuby2-P2A-PuroR CDS |
| 239697 | pKG1055_pPV2-tagBFP | BsaI Golden Gate assembly plasmid encoding the tagBFP CDS |
| 239698 | pKG3659_pPV2-rtTA-P2A-iRFP720 | BsaI Golden Gate assembly plasmid encoding the rtTA-P2A-iRFP720 CDS |
| 239699 | pKG3658_pPV2-NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 | BsaI Golden Gate assembly plasmid encoding the COMET NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 CDS |
| 239701 | pKG0587_pPV3-bGH | BsaI Golden Gate assembly plasmid encoding the bGH PAS |
| 239702 | pKG0588_pPV3-SV40 | BsaI Golden Gate assembly plasmid encoding SV40 PAS |
| 239703 | pKG1313_pPV3-WPRE | BsaI Golden Gate assembly plasmid encoding the WPRE 3' UTR sequence |
| 239705 | pKG1334_pHarbor-PiggyBac_backbone | PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration |
| 239706 | pKG3560_pHarbor-Rogi2_backbone | PaqCI Golden Gate assembly plasmid encoding the backbone for Bxb1-mediated integration in the Rogi2 landing pad line |
| 239707 | pKG1970_pGEEC501_pShip-EFS-mRuby2-bGH | Plasmid expressing mRuby2 with the EFS promoter and bGH 3' UTR |
| 239708 | pKG1971_pGEEC502_pShip-EFS-mRuby2-SV40 | Plasmid expressing mRuby2 with the EFS promoter and SV40 3' UTR |
| 239709 | pKG1972_pGEEC503_pShip-EFS-mRuby2-WPRE | Plasmid expressing mRuby2 with the EFS promoter and WPRE 3' UTR |
| 239710 | pKG1973_pGEEC504_pShip-hPGK-mRuby2-bGH | Plasmid expressing mRuby2 with the hPGK promoter and bGH 3' UTR |
| 239711 | pKG1974_pGEEC505_pShip-hPGK-mRuby2-SV40 | Plasmid expressing mRuby2 with the hPGK promoter and SV40 3' UTR |
| 239712 | pKG1975_pGEEC506_pShip-hPGK-mRuby2-WPRE | Plasmid expressing mRuby2 with the hPGK promoter and WPRE 3' UTR |
| 239713 | pKG2021_pGEEC507_pShip-UbC-mRuby2-bGH | Plasmid expressing mRuby2 with the UbC promoter and bGH 3' UTR |
| 239714 | pKG2022_pGEEC508_pShip-UbC-mRuby2-SV40 | Plasmid expressing mRuby2 with the UbC promoter and SV40 3' UTR |
| 239715 | pKG2023_pGEEC509_pShip-UbC-mRuby2-WPRE | Plasmid expressing mRuby2 with the UbC promoter and WPRE 3' UTR |
| 239716 | pKG2116_pGEEC510_pShip-EF1a-mRuby2-bGH | Plasmid expressing mRuby2 with the EF1a promoter and bGH 3' UTR |
| 239717 | pKG2117_pGEEC511_pShip-EF1a-mRuby2-SV40 | Plasmid expressing mRuby2 with the EF1a promoter and SV40 3' UTR |
| 239718 | pKG2118_pGEEC512_pShip-EF1a-mRuby2-WPRE | Plasmid expressing mRuby2 with the EF1a promoter and WPRE 3' UTR |
| 239719 | pKG2075_pGEEC516_pShip-CAG-mRuby2-bGH | Plasmid expressing mRuby2 with the CAG promoter and bGH 3' UTR |
| 239720 | pKG2076_pGEEC517_pShip-CAG-mRuby2-SV40 | Plasmid expressing mRuby2 with the CAG promoter and SV40 3' UTR |
| 239721 | pKG2077_pGEEC518_pShip-CAG-mRuby2-WPRE | Plasmid expressing mRuby2 with the CAG promoter and WPRE 3' UTR |
| 239722 | pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH | Plasmid expressing a grazoprevir-inducible transcriptional activator |
| 239723 | pKG2215_pGEEC525_pShip-ZF43x6-C-mRuby2-bGH | Plasmid expressing mRuby2 from a COMET promoter, Induced with pKG3666 |
| 239724 | pKG2410_pGEEC526_pShip-TRE3G-mRuby2-bGH | Plasmid expressing mRuby2 from the TRE3G promoter, Induced with pKG3668 |
| 239725 | pKG2497_pGEEC529_pShip-CMV-mRuby2-bGH | Plasmid expressing mRuby2 with the CMV promoter and bGH 3' UTR |
| 239726 | pKG2498_pGEEC530_pShip-CMV-mRuby2-SV40 | Plasmid expressing mRuby2 with the CMV promoter and SV40 3' UTR |
| 239727 | pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE | Plasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTR |
| 239728 | pKG2436_pGEEC532_pShip-EF1a-mRuby2-P2A-PuroR-bGH | Plasmid expressing mRuby2 and PuroR with the EF1a promoter |
| 239729 | pKG2437_pGEEC533_pShip-EFS-mRuby2-P2A-PuroR-bGH | Plasmid expressing mRuby2 and PuroR with the EFS promoter |
| 239730 | pKG2438_pGEEC534_pShip-CMV-mRuby2-P2A-PuroR-bGH | Plasmid expressing mRuby2 and PuroR with the CMV promoter |
| 239731 | pKG2439_pGEEC535_pShip-hPGK-mRuby2-P2A-PuroR-bGH | Plasmid expressing mRuby2 and PuroR with the hPGK promoter |
| 239732 | pKG2440_pGEEC536_pShip-UbC-mRuby2-P2A-PuroR-bGH | Plasmid expressing mRuby2 and PuroR with the UbC promoter |
| 239733 | pKG2441_pGEEC537_pShip-CAG-mRuby2-P2A-PuroR-bGH | Plasmid expressing mRuby2 and PuroR with the CAG promoter |
| 239734 | pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH | Plasmid expressing tagBFP with the EFS promoter |
| 239735 | pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH | Plasmid expressing tagBFP with the hPGK promoter |
| 239736 | pKG2897_pGEEC548_pShip-UbC-tagBFP-bGH | Plasmid expressing tagBFP with the UbC promoter |
| 239737 | pKG2899_pGEEC550_pShip-CMV-tagBFP-bGH | Plasmid expressing tagBFP with the CMV promoter |
| 239738 | pKG2900_pGEEC551_pShip-CAG-tagBFP-bGH | Plasmid expressing tagBFP with the CAG promoter |
| 239739 | pKG3660_pGEEC570_pShip-ZF10.BSx8-ybTATA-spacer-mRuby2-bGH | Plasmid expressing mRuby2 from a SynZiFTR promoter, Induced with pKG2309 |
| 239740 | pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH | Plasmid expressing a rapamycin-inducible transcriptional activator |
| 239741 | pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH | Plasmid expressing a doxycycline-inducible transcriptional activator |
| 239742 | pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH | Plasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA production |
| 239743 | pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH | Plasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA production |
| 239744 | pKG3775_pGEEC582_pShip-hPGK(promoter)-CMV(UTR)-mRuby2-bGH | Plasmid expressing mRuby2 from the hPGK canonical promoter with the CMV 5' UTR |
| 239745 | pKG3776_pGEEC583_pShip-hPGK(promoter)-EFS(UTR)-mRuby2-bGH | Plasmid expressing mRuby2 from the hPGK canonical promoter with the EFS 5' UTR |
| 239746 | pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH | Plasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTR |
| 239747 | pKG3778_pGEEC585_pShip-EFS(promoter)-hPGK(UTR)-mRuby2-bGH | Plasmid expressing mRuby2 from the EFS canonical promoter with the hPGK 5' UTR |
| 239748 | pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH | Plasmid expressing mRuby2 from the CMV canonical promoter with the EFS 5' UTR |
| 239749 | pKG3780_pGEEC587_pShip-CMV(promoter)-hPGK(UTR)-mRuby2-bGH | Plasmid expressing mRuby2 from the CMV canonical promoter with the hPGK 5' UTR |