A proximity-labeling-based approach to directly detect mRNA delivery to specific subcellular locations.
Smart AD, Hughes ME, Ruiz Velasco AD, Hori N, Stolnik S, Jopling CL
Mol Ther Nucleic Acids. 2025 Jun 24;36(3):102602. doi: 10.1016/j.omtn.2025.102602. eCollection 2025 Sep 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239753 | pNluc-hbb | Template for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239843 | pIL6-Nluc-hbb | Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239862 | pPMEL-Nluc-hbb | Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239863 | pPMEL-hbb | Template for in vitro transcription of PMEL (gp100), including native signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239868 | pIL6-PMEL-hbb | Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs. |