A proximity labelling-based approach to directly detect mRNA delivery to specific subcellular locations
Smart AD, Hughes ME, Ruiz Velasco AD, Hori N, Stolnik S, Jopling CL
Molecular Therapy Nucleic Acids, 102602. doi: 10.1016/j.omtn.2025.102602
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239753 | pNluc-hbb | Template for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239843 | pIL6-Nluc-hbb | Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239862 | pPMEL-Nluc-hbb | Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239863 | pPMEL-hbb | Template for in vitro transcription of PMEL (gp100), including native signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs. |
| 239868 | pIL6-PMEL-hbb | Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs. |