In vitro kinetic profiling by enzymatic approaches to select FAPIs with a long residence time.

Verhulst E, Brzeminski P, de Groot A, Wals JW, Michiels R, Fabisiak A, Thys J, Grintsevich S, Van Rymenant Y, Peeters S, Lambregts S, Sim Y, Verstraete R, Van Wielendaele P, Berg M, Elvas F, Van Ostade X, Rosch F, Lambeir AM, De Winter H, Van Der Veken P, De Meester I
Eur J Med Chem. 2026 Feb 15;304:118494. doi: 10.1016/j.ejmech.2025.118494. Epub 2025 Dec 18. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
239817	pHLsec-FAP	Expresses human fibroblast activation protein alpha (FAP) in mammalian cells