CRISPRi-based circuits to control gene expression in plants.
Khan MA, Herring G, Zhu JY, Oliva M, Fourie E, Johnston B, Zhang Z, Potter J, Pineda L, Pflueger J, Swain T, Pflueger C, Lloyd JPB, Secco D, Small I, Kidd BN, Lister R
Nat Biotechnol. 2025 Mar;43(3):416-430. doi: 10.1038/s41587-024-02236-w. Epub 2024 May 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239869 | LLP947 | Wild type TCTP promoter driving the expression of Renilla luciferase |
| 239870 | LLP948 | TCTP-SynPro-01 engineered promoter driving the expression of Renilla luciferase |
| 239871 | LLP949 | TCTP-SynPro-02 engineered promoter driving the expression of Renilla luciferase |
| 239872 | LLP950 | TCTP-SynPro-03 engineered promoter driving the expression of Renilla luciferase |
| 239873 | LLP951 | TCTP-SynPro-04 engineered promoter driving the expression of Renilla luciferase |
| 239874 | LLP952 | TCTP-SynPro-05 engineered promoter driving the expression of Renilla luciferase |
| 239875 | LLP953 | TCTP-SynPro-06 engineered promoter driving the expression of Renilla luciferase |
| 239876 | LLP954 | TCTP-SynPro-07 engineered promoter driving the expression of Renilla luciferase |
| 239877 | LLP955 | TCTP-SynPro-08 engineered promoter driving the expression of Renilla luciferase |
| 239878 | LLP956 | TCTP-SynPro-09 engineered promoter driving the expression of Renilla luciferase |
| 239879 | LLP957 | TCTP-SynPro-10 engineered promoter driving the expression of Renilla luciferase |
| 239880 | LLP958 | TCTP-SynPro-11 engineered promoter driving the expression of Renilla luciferase |
| 239881 | LLP959 | TCTP-SynPro-12 engineered promoter driving the expression of Renilla luciferase |
| 239882 | LLP960 | TCTP-SynPro-13 engineered promoter driving the expression of Renilla luciferase |
| 239883 | LLP961 | TCTP-SynPro-14 engineered promoter driving the expression of Renilla luciferase |
| 239884 | LLP962 | TCTP-SynPro-15 engineered promoter driving the expression of Renilla luciferase |
| 239885 | LLP963 | TCTP-SynPro-16 engineered promoter driving the expression of Renilla luciferase |
| 239886 | LLP964 | TCTP-SynPro-17 engineered promoter driving the expression of Renilla luciferase |
| 239887 | LLP965 | Wild type CaMV 35S promoter engineered promoter driving the expression of Renilla luciferase |
| 239888 | LLP966 | CaMV 35S-SynPro-01 engineered promoter driving the expression of Renilla luciferase |
| 239889 | LLP967 | CaMV 35S-SynPro-02 engineered promoter driving the expression of Renilla luciferase |
| 239890 | LLP968 | CaMV 35S-SynPro-03 engineered promoter driving the expression of Renilla luciferase |
| 239891 | LLP969 | CaMV 35S-SynPro-04 engineered promoter driving the expression of Renilla luciferase |
| 239892 | LLP970 | CaMV 35S-SynPro-05 engineered promoter driving the expression of Renilla luciferase |
| 239893 | LLP971 | CaMV 35S-SynPro-06 engineered promoter driving the expression of Renilla luciferase |
| 239894 | LLP972 | CaMV 35S-SynPro-07 engineered promoter driving the expression of Renilla luciferase |
| 239895 | LLP973 | CaMV 35S-SynPro-08 engineered promoter driving the expression of Renilla luciferase |
| 239896 | LLP974 | CaMV 35S-SynPro-09 engineered promoter driving the expression of Renilla luciferase |
| 239897 | LLP975 | CaMV 35S-SynPro-10 engineered promoter driving the expression of Renilla luciferase |
| 239898 | LLP976 | CaMV 35S-SynPro-11 engineered promoter driving the expression of Renilla luciferase |
| 239899 | LLP977 | CaMV 35S-SynPro-12 engineered promoter driving the expression of Renilla luciferase |
| 239900 | LLP978 | CaMV 35S-SynPro-13 engineered promoter driving the expression of Renilla luciferase |
| 239901 | LLP979 | CaMV 35S-SynPro-14 engineered promoter driving the expression of Renilla luciferase |
| 239902 | LLP980 | CaMV 35S-SynPro-15 engineered promoter driving the expression of Renilla luciferase |
| 239903 | LLP981 | TCTP-SynPro-01 as Level-0 part (Golden Gate cloning) |
| 239904 | LLP982 | TCTP-SynPro-02 as Level-0 part (Golden Gate cloning) |
| 239905 | LLP983 | TCTP-SynPro-03 as Level-0 part (Golden Gate cloning) |
| 239906 | LLP984 | TCTP-SynPro-04 as Level-0 part (Golden Gate cloning) |
| 239907 | LLP985 | TCTP-SynPro-05 as Level-0 part (Golden Gate cloning) |
| 239908 | LLP986 | TCTP-SynPro-06 as Level-0 part (Golden Gate cloning) |
| 239909 | LLP987 | TCTP-SynPro-07 as Level-0 part (Golden Gate cloning) |
| 239910 | LLP988 | TCTP-SynPro-08 as Level-0 part (Golden Gate cloning) |
| 239911 | LLP989 | TCTP-SynPro-09 as Level-0 part (Golden Gate cloning) |
| 239912 | LLP990 | TCTP-SynPro-10 as Level-0 part (Golden Gate cloning) |
| 239913 | LLP991 | sgRNA-A compatible with TCTP-SynPro-03, TCTP-SynPro-16 and CaMV 35S-SynPro-01 as Level-0 part (Goden Gate) |
| 239914 | LLP992 | sgRNA-B compatible with TCTP-SynPro-03, TCTP-SynPro-17 and CaMV 35S-SynPro-01 as Level-0 part (Goden Gate cloning) |
| 239915 | LLP993 | sgRNA-C compatible with TCTP-SynPro-01, TCTP-SynPro-17 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning) |
| 239916 | LLP994 | sgRNA-D compatible with TCTP-SynPro-01, TCTP-SynPro-16 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning) |
| 239917 | LLP995 | sgRNA-E compatible with TCTP-SynPro-04, TCTP-SynPro-06 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning) |
| 239918 | LLP996 | sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-15 as Level-0 part (Golden Gate cloning) |
| 239919 | LLP997 | sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-07 as Level-0 part (Golden Gate cloning) |
| 239920 | LLP998 | sgRNA compatible with TCTP-SynPro-06, TCTP-SynPro-13 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning) |
| 239921 | LLP999 | sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning) |
| 239922 | LLP1000 | sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning) |
| 239923 | LLP1001 | sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning) |
| 239924 | LLP1002 | sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning) |
| 239926 | LLP1004 | sgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-12 as Level-0 part (Golden Gate cloning) |
| 239927 | LLP1005 | sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning) |
| 239928 | LLP1006 | sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning) |
| 239929 | LLP1007 | sgRNA compatible with TCTP-SynPro-04, TCTP-SynPro-11 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning) |
| 239930 | LLP1008 | sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning) |
| 239931 | LLP1009 | sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning) |
| 239932 | LLP1010 | dCas9 with Stop codon as Level-0 part (Golden Gate cloning) |
| 239933 | LLP1011 | dCas9 without Stop codon as Level-0 part (Golden Gate cloning) |
| 239934 | LLP1012 | Csy4 as Level-0 part (Golden Gate cloning) |
| 239935 | LLP1013 | Csy4 recognition sequences (Cog) as Level-0 part (Golden Gate cloning) |
| 239936 | LLP1014 | Hsp18.2 (heat inducible) promoter driving the expression of sgRNA-B |
| 239937 | LLP1015 | CaMV 35S driving the expression of dCas9 |
| 239938 | LLP1016 | CaMV 35S driving the expression of dCas9-ZAT10(2x) |
| 239939 | LLP1017 | CaMV 35S driving the expression of dCas9-ZAT10(1x) |
| 239940 | LLP1018 | CaMV 35S driving the expression of dCas9 |
| 239941 | LLP1019 | CaMV 35S driving the expression of dCas9-ZAT10(1x) |
| 239942 | LLP1020 | AtACT2 promoter driving the expression of dCas9-ZAT10(2x) |
| 239943 | LLP1021 | Renilla luciferase as a Level-0 part (Golden Gate) |
| 239944 | LLP1022 | Firefly luciferase plus as a Level-0 part (Golden Gate) |
| 239945 | LLP1023 | CaMV 35S driving the expression of dCas9 |
| 239946 | LLP1024 | CaMV 35S-SynPro-01 engineered promoter driving the expression of Renilla luciferase |