A chimeric viral platform for directed evolution in mammalian cells.
Cole AJ, Denes CE, Moreno CL, Hunault L, Dobson T, Hesselson D, Neely GG
Nat Commun. 2025 May 7;16(1):4250. doi: 10.1038/s41467-025-59438-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240253 | pSFV_(3'UTR)_RLEloop | PROTEUS vector with no transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector. |
| 240254 | pSFV_eGFP-P2A-luc2pre (3'UTR) | Non-attenuated SFV vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector. |
| 240255 | pSFV_eGFP-P2A-luc2pre (3'UTR)_RLEloop | Attenuated PROTEUS vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector. |
| 240256 | pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop | Attenuated PROTEUS vector with Nb139-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector. |
| 240257 | pSFV_rTetR-VP16 (3'UTR)_RLEloop | Attenuated PROTEUS vector with rtTA-3G transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector. |
| 240258 | pSFV_SRF-NLS-VP64 (3'UTR)_RLEloop | Attenuated PROTEUS vector with SRF-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector. |
| 240259 | pSFV_TetR-VP16 (3'UTR)_RLEloop | Attenuated PROTEUS vector with tTA transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector. |
| 240260 | pSRE_G | Circuit-responsive (SRE promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during SRF-VP64 campaigns. |
| 240261 | pTRE3G_G | Circuit-responsive (TRE3G promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during tTA/rtTA-3G campaigns. |
| 240262 | pZF43x6-C_G | Circuit-responsive (ZF43RE promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during Nb139-VP64 campaigns. |
| 240263 | pCDNA3.1_p53-ZF43 | Constitutive expression (CMV promoter) of a fusion of the p53 DNA-binding domain and ZF43. Used as part of the circuit for Nb139-VP64 transgene activity. |
| 240264 | pcDNA3.1+_luciferase | Constitutive expression (CMV promoter) of firefly luciferase. Used as a control for luciferase assays. |
| 240265 | pcDNA3.1+_SRE_luciferase | Circuit-responsive (SRE promoter) expression of firefly luciferase. Used for evaluation of SRF-VP64 transgene activity. |
| 240266 | pcDNA3.1+_TRE3G_luciferase | Circuit-responsive (TRE3G promoter) expression of firefly luciferase. Used for evaluation of tTA/rtTA-3G transgene activity. |
| 240267 | pZF43x6_luciferase | Circuit-responsive (ZF43RE promoter) expression of firefly luciferase. Used for evaluation of Nb139-VP64 transgene activity. |