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Tim Reddy Lab: HyperCas12a enables highly-multiplexed epigenome editing screens.

Melore SM, Hamilton MC, Reddy TE
bioRxiv [Preprint]. 2024 Jul 9:2024.07.08.602263. doi: 10.1101/2024.07.08.602263. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
244204SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFPMammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker
244205SM266_EF1A-dHyperLbCas12a-KRAB-T2A-BlastRMammalian expression of dHyperLbCas12a-KRAB with Blasticidin resistance selectable marker
244206SM268_EF1A-dHyperLbCas12a-P300-T2A-EGFPMammalian expression of dHyperLbCas12a-P300 with EGFP selectable marker
244207SM269_EF1A-dHyperLbCas12a-P300-T2A-BlastRMammalian expression of dHyperLbCas12a-P300 with Blasticidin resistance selectable marker
244208SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFPMammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker
244210SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFPMammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker
244211SM273_EF1A-dHyperLbCas12a-VPR-T2A-BlastRMammalian expression of dHyperLbCas12a-VPR with Blasticidin resistance selectable marker
244212SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFPMammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker
244213SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastRMammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker
244214SM279_EF1A-dEnAsCas12a-4xSID-T2A-EGFPMammalian expression of dEnAsCas12a-4xSID with EGFP selectable marker
244215SM280_EF1A-dEnAsCas12a-4xSID-T2A-BlastRMammalian expression of dEnAsCas12a-4xSID with Blasticidin resistance selectable marker
244216SM281_EF1A-dEnAsCas12a-P300-T2A-EGFPMammalian expression of dEnAsCas12a-P300 with EGFP selectable marker
244217SM282_EF1A-dEnAsCas12a-P300-T2A-BlastRMammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker
244218SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFPMammalian expression of dEnAsCas12a-VPR with EGFP selectable marker
244219SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastRMammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker
244220SM468_EF1A-dHyperLbCas12a-T2A-EGFPMammalian expression of dHyperLbCas12a with EGFP selectable marker
244221SM469_EF1A-dHyperLbCas12a-T2A-BlastRMammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker
244222SM470_EF1A-dEnAsCas12a-T2A-EGFPMammalian expression of dEnAsCas12a with EGFP selectable marker
244223SM471_EF1A-dEnAsCas12a-T2A-BlastRMammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Antibodies from Article