C-terminal amides mark proteins for degradation via SCF-FBXO31.
Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek M, Schulman BA, Bode JW, Corn JE
Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250247 | pJC2281 - pHR-SFFV-rtTA3-PGK-Bsr | Expression of reverse tet transactivator for use in the Tet-On system. |
| 250251 | pJC2297 - pHR-TRE3G-hSpCas9-NLS-FLAG-2A-Thy1.1 | Expression of Cas9 with Thy1.1 cell surface marker under a doxycycline responsive promoter. Requires additional expression of transactivator. |
| 250254 | pJC2722 - pCDNA4TO-CMV-3xFLAG-Halo-AARS1 | cDNA expression of affinity-tagged AARS1. |
| 250255 | pJC2723 - pLenti-X1-EF1A-HA-FBXO31-PGK-Neo | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31). |
| 250256 | pJC2724 - pLenti-X1-EF1A-HA-FBXO31(∆F-box)-PGK-Neo | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box)). |
| 250257 | pJC2725 - pLenti-X1-EF1A-HA-FBXO31(Y309A)-PGK-Neo | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(Y309A)). |
| 250258 | pJC2726 - pLenti-X1-EF1A-HA-FBXO31(I337D)-PGK-Neo | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(I337D)). |
| 250259 | pJC2727 - pLenti-X1-EF1A-HA-FBXO31(T343V)-PGK-Neo | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(T343V)). |
| 250260 | pJC2728 - pLenti-X1-EF1A-HA-FBXO31(D334N)-PGK-Neo | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N)). |
| 250263 | pJC2731 - pHR-EF1A-HA-FBXO31-IRES-GFP | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31). |
| 250264 | pJC2732 - pHR-EF1A-HA-FBXO31(∆F-box)-IRES-GFP | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box)). |
| 250265 | pJC2733 - pHR-EF1A-HA-FBXO31(∆F-box, T343V)-IRES-GFP | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, T343V)). |
| 250266 | pJC2734 - pHR-EF1A-HA-FBXO31(D334N)-IRES-GFP | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N)). |
| 250267 | pJC2735 - pHR-EF1A-HA-FBXO31(∆F-box, D334N)-IRES-GFP | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, D334N)). |
| 250268 | pJC2737 - pHR-EF1A-DD-3xFLAG-FBXO31-IRES-GFP | Inducible expression of FBXO31 with the shield degron system. |
| 250269 | pJC2738 - pHR-EF1A-DD-3xFLAG-FBXO31(D334N)-IRES-GFP | Inducible expression of FBXO31(D334N) with the shield degron system. |
| 250271 | pJC2740 - pHR-EF1A-HA-FBXO31(D334N)-IRES-mTagBFP | cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N)) |
| 250272 | pJC2741 - hPGK-EGFP-CycD1(C-term)-IRES2-mCherry-EF1A-Puro | Ratiometric protein stability reporter for measuring C-terminal degron activity. |
| 250273 | pJC2742 - hPGK-EGFP-SUGT1(C-term)-IRES2-mCherry-EF1A-Puro | Ratiometric protein stability reporter for measuring C-terminal degron activity. |
| 250274 | pJC2743 - hPGK-EGFP-MTFR1L(C-term)-IRES2-mCherry-EF1A-Puro | Ratiometric protein stability reporter for measuring C-terminal degron activity. |
| 250275 | pJC2744 - hPGK-EGFP-GOPC(C-term)-IRES2-mCherry-EF1A-Puro | Ratiometric protein stability reporter for measuring C-terminal degron activity. |
| 250277 | pJC2746 - pLC-U6-spacer-modified-tracer-EFS-eBFP2-P2A-Puro | Empty backbone for cloning and stable sgRNA expression. |