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A CRISPR-Cas-associated transposon system for genome editing in Burkholderia cepacia complex species.

Yap ZL, Rahman ASMZ, Hogan AM, Levin DB, Cardona ST
Appl Environ Microbiol. 2024 Jul 24;90(7):e0069924. doi: 10.1128/aem.00699-24. Epub 2024 Jun 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
211787pZLrhaB2pluspSCrhaB2plus with modifications of resistance cassette to CmR
211788pDonorTppDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR
211790pDonorTp-fugfppDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites
211791pRhaCASTpZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter

Antibodies from Article