A CRISPR-Cas-associated transposon system for genome editing in Burkholderia cepacia complex species.
Yap ZL, Rahman ASMZ, Hogan AM, Levin DB, Cardona ST
Appl Environ Microbiol. 2024 Jul 24;90(7):e0069924. doi: 10.1128/aem.00699-24. Epub 2024 Jun 13.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
211787 | pZLrhaB2plus | pSCrhaB2plus with modifications of resistance cassette to CmR |
211788 | pDonorTp | pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR |
211790 | pDonorTp-fugfp | pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites |
211791 | pRhaCAST | pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter |