A CRISPR-Cas-associated transposon system for genome editing in Burkholderia cepacia complex species.
Yap ZL, Rahman ASMZ, Hogan AM, Levin DB, Cardona ST
Appl Environ Microbiol. 2024 Jul 24;90(7):e0069924. doi: 10.1128/aem.00699-24. Epub 2024 Jun 13.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211787 | pZLrhaB2plus | pSCrhaB2plus with modifications of resistance cassette to CmR |
| 211788 | pDonorTp | pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR |
| 211790 | pDonorTp-fugfp | pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites |
| 211791 | pRhaCAST | pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter |