Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenome.
Metzner E, Southard KM, Norman TM
Cell Syst. 2025 Jan 15;16(1):101161. doi: 10.1016/j.cels.2024.12.002. Epub 2024 Dec 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224898 | pEM040 [Multiome Perturb-seq sgRNA expression vector] | Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments |
| 224899 | pEM047 [T7-assisted CROPseq sgRNA expression vector] | Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA |