Multiplexed volumetric CLEM enabled by scFvs provides insights into the cytology of cerebellar cortex.

Han X, Lu X, Li PH, Wang S, Schalek R, Meirovitch Y, Lin Z, Adhinarta J, Murray KD, MacNiven LM, Berger DR, Wu Y, Fang T, Meral ES, Asraf S, Ploegh H, Pfister H, Wei D, Jain V, Trimmer JS, Lichtman JW
Nat Commun. 2024 Aug 5;15(1):6648. doi: 10.1038/s41467-024-50411-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
204419	anti-GFP scFv [N86/38] with sortase tag	Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.
204420	anti-GFAP R416WT scFv [N206B/9] with sortase tag	Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.
204421	anti-Calbindin scFv [L109/57] with sortase tag	Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.
204422	anti-PSD-95 scFv [K28/43] with sortase tag	Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.
204423	anti-Parvalbumin scFv [L114/81] with sortase tag	Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.
204424	anti-VGluT1 scFv [N28/9] with sortase tag	Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.
204425	anti-Kv1.2 scFv [K14/16] with sortase tag	Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16.
204426	anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag	Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.