Lysosomal TBK1 responds to amino acid availability to relieve Rab7-dependent mTORC1 inhibition.
Talaia G, Bentley-DeSousa A, Ferguson SM
EMBO J. 2024 Sep;43(18):3948-3967. doi: 10.1038/s44318-024-00180-8. Epub 2024 Aug 5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221550 | pSpCas9-2A-Puro-TBK1-gRNA (PX459) | Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells |
| 221551 | pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459) | Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells |
| 221552 | pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459) | Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells |
| 221553 | pPB-EF1A-Puro-hTBK1-EGFP | Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration. |
| 221554 | pPB-EF1A-Puro-hTBK1-E696K-EGFP | Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration. |
| 221555 | pPB-EF1A-Puro-mCherry-RAB7 | Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration. |
| 221556 | pPB-EF1A-Puro-mCherry-RAB7-S72A | Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration. |