Principles of CRISPR-Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision.
Shembrey C, Yang R, Casan J, Hu W, Chen H, Singh GJ, Sadras T, Prasad K, Shortt J, Johnstone RW, Trapani JA, Ekert PG, Fareh M
Sci Adv. 2024 Dec 20;10(51):eadl0731. doi: 10.1126/sciadv.adl0731. Epub 2024 Dec 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221019 | MSCV-KRAS-WT-IRES-mCherry | Fluorescent reporter for expressing the KRAS wildtype CDS |
| 221020 | MSCV-KRAS-G12A-IRES-mCherry | Fluorescent reporter for expressing the KRAS G12A mutant CDS |
| 221021 | MSCV-KRAS-G12C-IRES-mCherry | Fluorescent reporter for expressing the KRAS G12C mutant CDS |
| 221022 | MSCV-KRAS-G12D-IRES-mCherry | Fluorescent reporter for expressing the KRAS G12D mutant CDS |
| 221023 | MSCV-KRAS-G12R-IRES-mCherry | Fluorescent reporter for expressing the KRAS G12R mutant CDS |
| 221024 | MSCV-KRAS-G12S-IRES-mCherry | Fluorescent reporter for expressing the KRAS G12S mutant CDS |
| 221025 | MSCV-KRAS-G12V-IRES-mCherry | Fluorescent reporter for expressing the KRAS G12V mutant CDS |
| 221026 | MSCV-BRAF-V600E-IRES-mCherry | Fluorescent reporter for expressing a segment of BRAF-V600E CDS |
| 221027 | MSCV-BRAF-WT-IRES-mCherry | Fluorescent reporter for expressing a segment of wild type BRAF |