Rapid and quantitative functional interrogation of human enhancer variant activity in live mice.
Hollingsworth EW, Liu TA, Alcantara JA, Chen CX, Jacinto SH, Kvon EZ
Nat Commun. 2025 Jan 6;16(1):409. doi: 10.1038/s41467-024-55500-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211940 | PCR4-Hsp68::mCherry-H11 | dual-enSERT-1 mCherry vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter) |
| 211941 | PCR4-Hsp68::eGFP-H11 | dual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter) |
| 211942 | PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11 | dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator) |
| 216394 | PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11 | dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator) |