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Semaphorin heterodimerization in cis regulates membrane targeting and neocortical wiring.

Bessa P, Newman AG, Yan K, Schaub T, Dannenberg R, Lajko D, Eilenberger J, Brunet T, Textoris-Taube K, Kemmler E, Deng P, Banerjee P, Ravindran E, Preissner R, Rosario M, Tarabykin V
Nat Commun. 2024 Aug 16;15(1):7059. doi: 10.1038/s41467-024-51009-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190644pCAG-HA-Sema7AExpresses Mouse Sema7A with HA Tag
190645pCAG-sp-myc-mSema4DExpresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide
190646pCAG-Sema4D-FlagExpresses Mouse Sema4D with C Terminal Flag Tag
190647pCAGIG-Sema7A-ΔSEMAExpresses Mouse Sema7A with SEMA domain deleted
190648pCAGIG-Sema7A-ΔIGExpresses Mouse Sema7A with IG Domain Deleted
190649pCAGIG-Sema7A-ΔPSIExpresses Mouse Sema7A with PSI domain deleted
190651pCAGIG-Sema7A-KCEExpresses Mouse Sema7A with RGD motif mutated to KCE
190654pCAG-sp-myc-hSema4DExpresses Human Sema4D with N terminal myc after signal peptide
190655pCAG-sp-myc-hSema4D-Q497PExpresses Human Sema4D with N terminal myc after signal peptide containing 497P mutation
190656pCAG-sp-flag-hSema4DExpresses Human Sema4D with N terminal flag after signal peptide
190657pCAG-sp-flag-hSema4D-Q497PExpresses Human Sema4D with N terminal flag after signal peptide containing 497P mutation

Antibodies from Article