SINE compounds activate exportin 1 degradation through an allosteric mechanism
Wing CE, Fung HYJ, Kwanten B, Cagatay T, Niesman AB, Jacquemyn M, Gharghabi M, Permentier B, Shakya B, Nandi R, Ready JM, Kashyap T, Shacham S, Landesman Y, Lapalombella R, Daelemans D, Chook YM
Nat Chem Biol (2025) 21, 2002–2013.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 242459 | pGL4-CMV-FLAG-XPO1 | Expresses human XPO1 with an N-terminal FLAG tag for pull-down assays. |
| 242460 | pLV-puro-EF1A-Tag100-hASB8 | Expresses human ASB8 protein with an N-terminal Tag100 along with a puromycin selection marker. |
| 242461 | pLV-puro-EF1A-Tag100-hASB8_G198R | Expresses human ASB8 protein with an N-terminal Tag100 along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding. |
| 242463 | pLCKO-CMV-cMyc-UBC_K7R-IRES-Blasti | Expresses human UBC protein with an N-terminal cMyc tag enabling UBC pull down along with a blasticidin selection marker. The K7R mutations prevent ubiquitin chain elongation. |
| 242464 | pLV-puro-EF1A-3XFLAG-hASB8 | Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. |
| 242465 | pLV-puro-EF1A-3XFLAG-hASB8_G198R | Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding. |
| 242466 | pLV-puro-EF1A-3XFLAG-hASB8_L199R | Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199R mutation disrupts XPO1 binding. |
| 242467 | pLV-puro-EF1A-3XFLAG-hASB8_L199G | Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199G mutation disrupts XPO1 binding. |
| 242468 | pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4 | Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The ANK3-4 mutations disrupt XPO1 binding. |
| 242469 | pGL4-CMV-myc-XPO1 | Expresses human XPO1 protein with an N-terminal cMyc tag. |
| 242470 | pGL4-CMV-myc-XPO1_V518E_F561H | Expresses human XPO1 protein with an N-terminal cMyc tag. The V518E_5561H mutations disrupt ASB8 binding. |
| 242471 | pGL4-CMV-myc-XPO1_H10-11 | Expresses human XPO1 protein with an N-terminal cMyc tag. The H10-11 mutations disrupt ASB8 binding. |