Georgios Karras Lab: Protein-folding chaperones predict structure-function relationships and cancer risk in BRCA1 mutation carriers.
Gracia B, Montes P, Gutierrez AM, Arun B, Karras GI
Cell Rep. 2024 Feb 27;43(2):113803. doi: 10.1016/j.celrep.2024.113803. Epub 2024 Feb 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249006 | pKlab003-pcDNA3.1-FKBP5-3xFLAG | This plasmid expresses the C terminally 3x-FLAG tagged FKBP5 protein sequence for pulldown assays |
| 249008 | pKlab525-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-WT-STOP | This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain |
| 249009 | pKlab521-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-Y1845F-STOP | This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the Y1845F mutation |
| 249010 | pKlab522-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-Y1845D-STOP | This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the Y1845D mutation |
| 249011 | pKlab524-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-G1788V-STOP | This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the G1788V mutation |
| 249012 | pKlab518-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-A1708E-STOP | This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the A1708E mutation |
| 249013 | pKlab187-pcDNA3-BRCA1(1314-end)-WT-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain |
| 249014 | pKlab979-pcDNA3-BRCA1(1314-end)-A1708V-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the A1708V mutation |
| 249015 | pKlab211-pcDNA3-BRCA1(1314-end)-L1705R-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the L1705R mutation |
| 249016 | pKlab240-pcDNA3-BRCA1(1314-end)-Y1845D-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the Y1845D mutation |
| 249018 | pKlab981-pcDNA3-BRCA1(1314-end)-V1736A-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the V1736A mutation |
| 249019 | pKlab224-pcDNA3-BRCA1(1314-end)-R1699W-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the R1699W mutation |
| 249020 | pKlab223-pcDNA3-BRCA1(1314-end)-R1699Q-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the R1699Q mutation |
| 249021 | pKlab228-pcDNA3-BRCA1(1314-end)-S1715R-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the S1715R mutation |
| 249022 | pKlab189-pcDNA3-BRCA1(1314-end)-A1708E-V5-3xFLAG | This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the A1708E mutation |