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Polysome collapse and RNA condensation fluidize the cytoplasm.

Xie Y, Shu T, Liu T, Spindler MC, Mahamid J, Hocky GM, Gresham D, Holt LJ
Mol Cell. 2024 Jul 25;84(14):2698-2716.e9. doi: 10.1016/j.molcel.2024.06.024. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
240101pUBC-popTag-QtE-mScarletMammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.
240102pHR-PUM.HD-mCherry-Cry2WTMammalian lentiviral vector for expressing the mCherry-tagged Pumilio homology domain fused to the light-inducible oligomerization domain CRY2.

Antibodies from Article