Poltheta is phosphorylated by PLK1 to repair double-strand breaks in mitosis.
Gelot C, Kovacs MT, Miron S, Mylne E, Haan A, Boeffard-Dosierre L, Ghouil R, Popova T, Dingli F, Loew D, Guirouilh-Barbat J, Del Nery E, Zinn-Justin S, Ceccaldi R
Nature. 2023 Sep;621(7978):415-422. doi: 10.1038/s41586-023-06506-6. Epub 2023 Sep 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249811 | PiggyBac CAG eGFP-PolQ-FLAG P2A-BLAST | Expresses the PolQ (polymerase theta) protein fused with N-terminal eGFP and C-terminal FLAG tag |
| 249812 | PiggyBac CAG eGFP-PolQ10A-FLAG P2A-BLAST | Expresses the polymerase theta protein fused with N-terminal eGFP and C-terminal FLAG tag and mutated at serines 1289, 1482, 1486, 1488, 1493, 1555, 1563, 1628, 1635 and threonine 1755 into alanines |