A facile chemical strategy to synthesize precise AAV-protein conjugates for targeted gene delivery.

Pham Q, Glicksman J, Han B, Shahraeini SS, Jewel D, Loynd C, Singha Roy SJ, Chatterjee A
Mol Ther Oncol. 2025 Aug 23;33(4):201040. doi: 10.1016/j.omton.2025.201040. eCollection 2025 Dec 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
240122	pIDTsmart-RC2-delVP1-VP1-588TAG	Express AAV2 capsid with ncAA mutant at site 588 of VP1
240123	pIDTsmart-RC2-KO-delVP1-VP1-588TAG	Express AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 588 of VP1
240124	pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP	Express AAV2 transgene (EGFP) and MbPylRS/tRNA for ncAA incorporation