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Iterative in vivo cut’n’paste of chromosomal loci in Escherichia coli K-12 using synthetic DNA

Gude S, Bertelsen ABB, Nørholm MHH
bioRxiv 2025.06.19.660532 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
240153pRedCas9Expresses lambda red machinery and cas9
240154pCut_GFPExpresses CRISPR guides
240155pI-CreIAraRed_SC101_SpecExpresses lambda red machinery and i-creI

Antibodies from Article