Protein-based tools for the detection and characterisation of Oropouche virus infection.
Merchant MK, de Paula Souza J, Abdelkarim S, Chakraborty S, Nasser Neto TA, Gutierrez Manchay KI, de Melo Jorge DM, do Nascimento VA, Sun Y, Caroe ER, Eyssen LEA, Rudd JS, Ribeiro Piauilino IC, Damasceno Pinto S, Pereira da Silva MH, Rocha do Nascimento F, Naveca FG, Proenca-Modena JL, da Silva LLP, Pinto de Figueiredo RM, Owens RJ, Arruda E, Graham SC
EMBO Mol Med. 2025 Aug 11. doi: 10.1038/s44321-025-00291-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229662 | PB OROV Gc Spike H6 | Make PiggyBac stable cell line expressing Oropouche virus BeAn 19991 Gc spike region (residues 482–894 of M polyprotein) with C-terminal His6 tag |
| 229663 | pOPTH OROV N | Express Oropouche virus BeAn 19991 nucleoprotein with N-terminal His6 tag |
| 240162 | pMWCAVI_H6BAP-OROV_N | Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli |