Metabolite-responsive scaffold RNAs for dynamic CRISPR transcriptional regulation.
Stohr AM, Hansen H, Richards B, Park H, Goncalves AG, Agrawal A, Blenner M, Chen W
Nucleic Acids Res. 2025 Nov 13;53(21):gkaf1290. doi: 10.1093/nar/gkaf1290.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240203 | pColE1-MP-mRFP1 | Medium copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity |
| 240204 | pSC101-MP-mRFP1 | Low copy mRFP1 reporter for monitoring metabolite-responsive CRISPRa activity |
| 240205 | pColE1-MP-Violacein | Violacein biosynthesis pathway induced by CRISPRa |
| 240207 | pCRISPRa-Theo-1 | Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a theophylline-responsive MR-scRNA |
| 240208 | pSpRYa-Theo-1 | Bacterial SpRYa vector expressing dSpRY, MCP-SoxS, and a theophylline-responsive MR-scRNA |
| 240209 | pCRISPRa-Trp | Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a tryptophan-responsive MR-scRNA |
| 240210 | pSC-pGAL10-CRISPRa | Yeast vector for integration of pGAL10-dCas9 and pGAL10-MCP-VPR at LEU2 locus |
| 240211 | pSC-MRscRNA | Yeast vector expressing theophylline-responsive MR-scRNA |
| 240212 | pHS-dCas9 | Mammalian vector for transient expression of dCas9 |
| 240213 | pHS-MCP-VPR | Mammalian vector for transient expression of MCP-VPR |
| 240214 | pHS-MRscRNA | Mammalian vector for transient expression of theophylline-responsive MR-scRNA with mCherry transfection marker |