Epithelial tension controls intestinal cell extrusion.
Krueger D, Spoelstra WK, Mastebroek DJ, Kok RNU, Wu S, Nikolaev M, Bannier-Helaouet M, Gjorevski N, Lutolf M, van Es J, van Zon J, Tans SJ, Clevers H
Science. 2025 Sep 4;389(6764):eadr8753. doi: 10.1126/science.adr8753. Epub 2025 Sep 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240798 | p2T-TetON-Hygro-Arhgef11-Cry2-mCh | Dox-inducible expression of Arhgef11-Cry2-mCherry |
| 240799 | pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX | CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem |
| 240800 | pCRISPR-HOT-mNeon-Stop-PGK-mCherry-CAAX | CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via PGK promoter |
| 240801 | p2T-CMV-H2B-eGFP-SV40-Hygro | Constitutive expression of H2B-eGFP |
| 240802 | p2T-H2B-iRFP-IRES-Blast | Constitutive expression of H2B-iRFP |
| 240804 | p2T-CAG-CIBN-eGFP-CAAX-Puro | Constitutive expression of CIBN-eGFP |