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An all-in-one plant virus-based vector toolkit for streamlined gene silencing, overexpression, and genome editing.

Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, Song G
Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
234896pVSeAll-in-one plant virus expression vector for virus-induced gene silencing (VIGS) and virus-mediated overexpression (VOX)
234897pVS-GhPDSThis all-in-one vector is used to specifically silence the GhPDS gene via virus-induced gene silencing (VIGS).
234898pVS-GhCHLIThis all-in-one vector is used to specifically silence the GhCHLI gene via virus-induced gene silencing (VIGS).
234899pVS-GhSPThis all-in-one vector is used to specifically silence the GhSP gene via virus-induced gene silencing (VIGS).
234902pVM-SFT-SPThis all-in-one vector is used to overexpress the GhSFT gene via virus-mediated overexpression (VOX), and specifically silence the GhSP gene via virus-induced gene silencing (VIGS), simultaneously.
234903pVS-GhOMT1_1This all-in-one vector is used to specifically silence the GhOMT1 gene via virus-induced gene silencing (VIGS).
234904pVS-GhOMT1_2This all-in-one vector is used to specifically silence the GhOMT1 gene via virus-induced gene silencing (VIGS).
234905pVS2eAll-in-one plant virus expression vector for virus-induced gene silencing (VIGS).
234906pVS2-GhCLAThis all-in-one vector is used to specifically silence the GhCLA gene via virus-induced gene silencing (VIGS).
234907pVS2-GoPGFThis all-in-one vector is used to specifically silence the GoPGF gene via virus-induced gene silencing (VIGS).
234908pVS2-NbPDSThis all-in-one vector is used to specifically silence the NbPDS gene via virus-induced gene silencing (VIGS).
234909pVM2-GoPGF-HD1This all-in-one vector is used to specifically silence the GoPGF and HD1 gene via virus-induced gene silencing (VIGS), simultaneously.
234913pVTcAll-in-one plant virus vector to detect circular genomes.
234914pVTAll-in-one plant virus expression vector for virus-assisted transient expression (VATE).
234915pVT-LUCThis all-in-one vector is used to express the LUC gene
234917pVT-EGFPThis all-in-one vector is used to express the EGFP gene
234920pVTCL2All-in-one subcellar localization vector. Construct containing EGFP tag and plasma membrane-targeted mCherry for co-localization study in plants.
234921pVTCL3All-in-one subcellar localization vector. Construct containing EGFP tag and cell endoplasmic reticulum-targeted mCherry for co-localization study in plants.
234922pVTCL4All-in-one subcellar localization vector. Construct containing EGFP tag and Golgi-targeted mCherry for co-localization study in plants.
234923pVTCL1-AtbZIP63This all-in-one subcellar localization vector is used to study the nuclear localization of AtbZIP63
234924pVTHV3All-in-one bimolecular fluorescence complementation (BiFC) vector
234925pVTHV3-(AtbZIP63-nV+cV)This all-in-one bimolecular fluorescence complementation (BiFC) vector is used to study the self-interation of AtbZIP63 proteins
234926pVTHV3-(nV+AtbZIP63-cV)This all-in-one bimolecular fluorescence complementation (BiFC) vector is used to study the self-interation of AtbZIP63 proteins
234927pVTHV3-(AtbZIP63-nV+AtbZIP63-cV)This all-in-one bimolecular fluorescence complementation (BiFC) vector is used to study the self-interation of AtbZIP63 proteins
234928pVEeAll-in-one plant virus expression vector for virus-induced genome editing (VIGE).
234929pVE-GhCLAThis all-in-one vector is used to specifically knock out the GhCLA gene via virus-induced genome editing (VIGE).
234931pVSr-GhPDSThis virus-induced gene silencing (VIGS) reporter vector is used to evaluate the silencing of GhPDS gene

Antibodies from Article