A novel approach towards a histone replacement system in Tetrapods
Pfisterer M, Pritz A, Parry A, Schmitz ML
PLoS One 21(2): e0342014 (2026)
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240416 | pHREP-KD-1 | Sleeping Beauty vector for inducible expression of an shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 240417 | pHREP-KD-1-shH2B | Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 240419 | pHREP-RE-1-H2B | Sleeping Beauty vector for the expression of a polycistronic mRNA with shRNA resistant chicken H2B and Blasticidin resistance |
| 240431 | pHREP-KD-2-shH4 | Sleeping Beauty vector for inducible expression of a chicken H4 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 240432 | pHREP-LP | Empty landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection. |
| 240433 | pHREP-LP-1xH2B | Landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection and 1x inducible expression cassette for shRNA resistant chicken H2B. |
| 240438 | pHREP-LP-2xH2B | Landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection and 2x inducible expression cassette for shRNA resistant chicken H2B. |
| 240439 | pHREP-LP-3xH2B | Landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection and 3x inducible expression cassette for shRNA resistant chicken H2B. |
| 240440 | pHREP-LP-4xH2B | Landing pad vector with two Bxb1 recombination sites for RMCE and LNGFR + EGFP-HygroR for sorting/selection and 4x inducible expression cassette for shRNA resistant chicken H2B. |
| 240441 | pHREP-KD-2 | Sleeping Beauty vector for inducible expression of an shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 240580 | pHREP-KD-2-shCtrl | Sleeping Beauty vector for inducible expression of a unspecific control shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 241994 | pHREP-KD-2-shH1 | Sleeping Beauty vector for inducible expression of a chicken H1 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 241995 | pHREP-KD-2-shH3 | Sleeping Beauty vector for inducible expression of a chicken H3 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 241996 | pHREP-KD-2-shH2B | Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |
| 241997 | pHREP-KD-2-shH2A | Sleeping Beauty vector for inducible expression of a chicken H2A targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin. |