Self-reporting Arabidopsis expressing pH and [Ca2+] indicators unveil ion dynamics in the cytoplasm and in the apoplast under abiotic stress.

Gao D, Knight MR, Trewavas AJ, Sattelmacher B, Plieth C
Plant Physiol. 2004 Mar;134(3):898-908. doi: 10.1104/pp.103.032508. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
240450	pBI121::chitGFP5:AQ	Expression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. For details, see Comments.
240451	pBI121::chitRatioGFP:LAAQ	Expression of indicator protein fusion (modified ratiometric pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. For details, see Comments.
240452	pBI121::chitEclipGFP:LAAQ	Expression of indicator protein fusion (modified ecliptic pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. For details, see Comments.
240453	pBI121::smRatioGFP:AQ	Expression of indicator protein fusion (soluble modified ratiometric pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. For details, see Comments.
240454	pBI121::smEclipGFP:AQ	Expression of indicator protein fusion (soluble modified ecliptic pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. For details, see Comments.