Optimized pipeline and designer cells for synthetic-biology-based high-throughput screening of viral protease inhibitors.
Edri S, El-Atawneh S, Ernst T, Elnekave M, Katzman C, Lanton T, Aldar I, Wolk O, Stern N, Goldblum A, Nissim L
Cell Rep Methods. 2025 Aug 5:101139. doi: 10.1016/j.crmeth.2025.101139.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240455 | pSE152: pFuGW-hUbCp-3CLpro-SV40NLS | SARS-CoV-2 Protease 3CLpro expression plasmid |
| 240456 | pTE3: pFuGW-SSX1p-GAL4BD-1X3CLpro consensus CS-VP16AD-SV40NLS | A synthetic transcription factor (synTF) that contains concensus 3CLpro cleavage site (CS) |
| 240457 | pSE163: pFuGW-SSX1p-GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS | A synthetic transcription factor (synTF) that contains a synthetic general 3CLpro cleavage site (CS) |
| 240460 | pTE5: pFuGW-SSX1p- GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS | a synthetic transcription factor (synTF) that contains 4 tandem synthetic general 3CLpro cleavage site (CS). |
| 240461 | pSE89: pFuGW-G5p-EYFP | Synthetic GAL4p comprising 5 tandem GAL4 binding sites (G5p) regulating EYFP |
| 240462 | pSE92: pFuGW-G14p-EYFP | A synthetic GAL4 promoter comprising 14 tandem GAL4 binding sites (G14p) regulating EYFP |