Absolute Quantification of Mammalian MicroRNAs for Therapeutic RNA Cleavage and Detargeting
Kraus C, Wang J, Zheng H, Broderick J, Ajaykumar N, Zamani M, Yang M, Cecchini K, Liang SQ, Kolumba O, Chase K, Lee J, Xue W, Sontheimer E, Gainetdinov I
RNA. 2025 May 28:rna.080566.125. doi: 10.1261/rna.080566.125.
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240513 | pEJS2783 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-16 CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 240514 | pEJS2784 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-20a CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 240515 | pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 240516 | pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 240517 | pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control. |
| 240518 | pEJS2782 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP | Mammalian expression of AcrIIC3-mCherry fusion with restriction enzyme sites in the 3' UTR for use as microRNA repression. BFP expressed separately as a transfection control. |