Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins.
Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins SE, Conklin BR, Nunez JK
bioRxiv [Preprint]. 2024 Nov 28:2024.11.26.625496. doi: 10.1101/2024.11.26.625496.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240527 | pCMV-MMLVgag-dCas9-ZIM3 | Expresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3 |
| 240528 | pCMV-MMLVgag-dCas9-KOX1 | Expresses MMLVgag–dCas9-KOX1 for producing RENDER-dCas9-KOX1 |
| 240530 | pCMV-MMLVgag-D3A-3L-dCas9 | Expresses MMLVgag–DNMT3A-3L-dCas9 for producing RENDER-DNMT3A-3L-dCas9 |
| 240531 | pCMV-MMLVgag-D3A-3L-dCas9-ZIM3 | Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing RENDER-DNMT3A-3L-dCas9-ZIM3 |
| 240532 | pCMV-MMLVgag-D3A-3L-dCas9-KOX1 | Expresses MMLVgag–DNMT3A-3L-dCas9-KOX1 for producing RENDER-DNMT3A-3L-dCas9-KOX1 |
| 240637 | mU6-sgRNA-CLTA-mCherry | Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter. |