Fibroblasts promote osmotic surveillance by wound-induced unique calcium patterns.
Fazekas L, Kaszas D, Vamosi B, Tamas SX, Szollosi T, Mihalyi V, Dehne FG, Vago-Kiss K, Al-Sheraji NM, Paulovits B, Roux BT, Enyedi B
J Cell Biol. 2026 Jan 5;225(1):e202501165. doi: 10.1083/jcb.202501165. Epub 2025 Oct 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240523 | pDestTol2CG2-itga11a-cFos:QF2 | Expresses QF2 in the fibroblasts of zebrafish along with the cardiac cmlc2:EGFP transgenesis marker |
| 240524 | p5E itga11a-cFos | 5' Gateway Entry vector containing the itga11a-cFos promoter for fibroblast-specific expression in zebrafish |
| 241912 | pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS | Zebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker |
| 241913 | pDestTol2CG2-QUAS:cPla2-mKate2 | Zebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac transgenesis marker |
| 241914 | pDestTol2CR-QUAS:NTR-mKate2 | Zebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker |
| 241915 | pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS | Zebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker |