Prime editor-based high-throughput screening reveals functional synonymous mutations in human cells.

Niu X, Tang W, Liu Y, Mo B, Yu Y, Liu Y, Wei W
Nat Biotechnol. 2025 Jun 24. doi: 10.1038/s41587-025-02710-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
240534	pLenti-CMV-PEmax-P2A-EGFP	For lentiviral expression of PEmax with EGFP
240538	pLenti-U6-epegRNA-SV40-mCherry	Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.