Prime editor-based high-throughput screening reveals functional synonymous mutations in human cells.
Niu X, Tang W, Liu Y, Mo B, Yu Y, Liu Y, Wei W
Nat Biotechnol. 2025 Jun 24. doi: 10.1038/s41587-025-02710-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240534 | pLenti-CMV-PEmax-P2A-EGFP | For lentiviral expression of PEmax with EGFP |
| 240535 | pLenti-U6-epegRNA eBAR1-SV40-puro | Lentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI. |
| 240536 | pLenti-U6-epegRNA eBAR2-SV40-puro | Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI. |
| 240537 | pLenti-U6-epegRNA eBAR3-SV40-puro | Lentiviral eBAR3 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI. |
| 240538 | pLenti-U6-epegRNA-SV40-mCherry | Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI. |
| 240539 | pLenti-U6-epegRNA-SV40-puro | Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI. |