Genome-wide in vivo and ex vivo mapping of R-loops using engineered N-terminal hybrid-binding domain of RNase H3 (enDR3).
Jedynak-Slyvka M, Kaczmarska Z, Graczyk D, Jurkiewicz A, Figiel M, Borowski LS, Szczesny RJ, Nowotny M
Nucleic Acids Res. 2025 Aug 11;53(15):gkaf792. doi: 10.1093/nar/gkaf792.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240546 | enDR3-TEV-mEGFP-NLS (pRS1451) | Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion. |
| 240547 | nb-enDR3-TEV-mEGFP-NLS (pRS1458) | Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS. |