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Catch & Release—rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach

Schwartz S, Engstler C, Mühlbauer S, Windenbach E, Wunder T, Kunz H, Brandt B
The Plant Journal, Volume124, Issue3, e70544, November 2025 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
240650pG20_HygExpression plasmid for un-tagged proteins with Hygromycin transgenic seed selection
240651pG20_TEV_mVenus_HygExpression plasmid for TEV-mVenus-tagged proteins with Hygromycin transgenic seed selection
240652pG20_TEV_mCherry_HygExpression plasmid for TEV-mCherry-tagged proteins with Hygromycin transgenic seed selection
240653pG20_mCherry_TEV_mVenus_HygExpression plasmid for mCherry-TEV-mVenus-tagged proteins with Hygromycin transgenic seed selection
240654pG20_3C_mCherry_TEV_mVenus_HygExpression plasmid for 3C-mCherry-TEV-mVenus-tagged proteins with Hygromycin transgenic seed selection
240655pG20_TEV_2xStrepII_HygExpression plasmid for TEV-2xStrepII-tagged proteins with Hygromycin transgenic seed selection
240656pG20_TEV_MYC_HygExpression plasmid for TEV-MYC-tagged proteins with Hygromycin transgenic seed selection
240657pG20_TEV_HA_HygExpression plasmid for TEV-HA-tagged proteins with Hygromycin transgenic seed selection
240658pG20_TEV_Flag_HygExpression plasmid for TEV-Flag-tagged proteins with Hygromycin transgenic seed selection
240659pG20_3C_mVenus_HygExpression plasmid for 3C-mVenus-tagged proteins with Hygromycin transgenic seed selection
240660pG20_FRExpression plasmid for un-tagged proteins with FAST-Red transgenic seed selection
240661pG20_TEV_mVenus_FRExpression plasmid for TEV-mVenus-tagged proteins with FAST-Red transgenic seed selection
240662pG20_TEV_mCherry_FRExpression plasmid for TEV-mCherry-tagged proteins with FAST-Red transgenic seed selection
240663pG20_mCherry_TEV_mVenus_FRExpression plasmid for mCherry-TEV-mVenus-tagged proteins with FAST-Red transgenic seed selection
240664pG20_3C_mCherry_TEV_mVenus_FRExpression plasmid for 3C-mCherry-TEV-mVenus-tagged proteins with FAST-Red transgenic seed selection
240665pG20_TEV_2xStrepII_FRExpression plasmid for TEV-2xStrepII-tagged proteins with FAST-Red transgenic seed selection
240666pG20_TEV_MYC_FRExpression plasmid for TEV-MYC-tagged proteins with FAST-Red transgenic seed selection
240667pG20_TEV_HA_FRExpression plasmid for TEV-HA-tagged proteins with FAST-Red transgenic seed selection
240668pG20_TEV_Flag_FRExpression plasmid for TEV-Flag-tagged proteins with FAST-Red transgenic seed selection
240669pG20_3C_mVenus_FRExpression plasmid for 3C-mVenus-tagged proteins with FAST-Red transgenic seed selection
240670pG20_FGExpression plasmid for un-tagged proteins with FAST-Green transgenic seed selection
240671pG20_TEV_mVenus_FGExpression plasmid for TEV-mVenus-tagged proteins with FAST-Green transgenic seed selection
240672pG20_TEV_mCherry_FGExpression plasmid for TEV-mCherry-tagged proteins with FAST-Green transgenic seed selection
240673pG20_mCherry_TEV_mVenus_FGExpression plasmid for mCherry-TEV-mVenus-tagged proteins with FAST-Green transgenic seed selection
240674pG20_3C_mCherry_TEV_mVenus_FGExpression plasmid for 3C-mCherry-TEV-mVenus-tagged proteins with FAST-Green transgenic seed selection
240675pG20_TEV_2xStrepII_FGExpression plasmid for TEV-2xStrepII-tagged proteins with FAST-Green transgenic seed selection
240676pG20_TEV_MYC_FGExpression plasmid for TEV-MYC-tagged proteins with FAST-Green transgenic seed selection
240677pG20_TEV_HA_FGExpression plasmid for TEV-HA-tagged proteins with FAST-Green transgenic seed selection
240678pG20_TEV_Flag_FGExpression plasmid for TEV-Flag-tagged proteins with FAST-Green transgenic seed selection
240679pG20_3C_mVenus_FGExpression plasmid for 3C-mVenus-tagged proteins with FAST-Green transgenic seed selection

Antibodies from Article