Genome editing of phylogenetically distinct bacteria using portable retron-mediated recombineering
González-Delgado A, Bonillo-Lopez L, Johnson MS, Knödlseder N, Ko CC, Lekbach Y, Oh JH, Selvakumar H, Wold MC, Yu Z, Aragón V, Gralnick JA, Güell M, Hatfull GF, Keitz BK, Koskella B, Mutalik VK, van Pijkeren JP, Shipman SL
bioRxiv 2025.06.16.660010
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240680 | pREC1010-Eco1 | RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240681 | pREC1010-Eco2 | RSF1010 origin of replication plasmid containing Eco2 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240682 | pREC1010-Ban1 | RSF1010 origin of replication plasmid containing Ban1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240683 | pREC1010-Abe1 | RSF1010 origin of replication plasmid containing Abe1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240684 | pREC1010-Kva1 | RSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240685 | pREC1010-Eas1 | RSF1010 origin of replication plasmid containing Eas1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240686 | pREC1010-Csp1 | RSF1010 origin of replication plasmid containing Csp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240687 | pREC1010-Tsp1 | RSF1010 origin of replication plasmid containing Tsp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240688 | pREC1010-Ebu1 | RSF1010 origin of replication plasmid containing Ebu1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240689 | pREC1010-Hcl1 | RSF1010 origin of replication plasmid containing Hcl recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter |
| 240690 | pREC1010C-Eco1 | RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter |
| 240691 | pREC1010lac-Eco1 | RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240692 | pREC1010lac-Eco2 | RSF1010 origin of replication plasmid containing Eco2 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240693 | pREC1010lac-Ban1 | RSF1010 origin of replication plasmid containing Ban1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240695 | pREC1010lac-Kva1 | RSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240696 | pREC1010lac-Eas1 | RSF1010 origin of replication plasmid containing Eas1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240697 | pREC1010lac-Csp1 | RSF1010 origin of replication plasmid containing Csp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240698 | pREC1010lac-Tsp1 | RSF1010 origin of replication plasmid containing Tsp1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240699 | pREC1010lac-Ebu1 | RSF1010 origin of replication plasmid containing Ebu1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240700 | pREC1010lac-Hcl1 | RSF1010 origin of replication plasmid containing Hcl recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter |
| 240701 | pREC1010-opEas1 | RSF1010 origin of replication plasmid containing Eas1 recombitron with extended a1 a2 regions in the ncRNA targeting lacZ locus in Klebsiella pneumoniae ATCC 10031 expressed by Pm promoter |
| 240702 | pREC1010-opEbu1 | RSF1010 origin of replication plasmid containing Ebu1 recombitron with extended a1 a2 regions with a donor in the ncRNA targeting lacZ locus in Citrobacter freundii ATCC 8090 expressed by Pm promoter |
| 240703 | pRECBRR1-Kva1 | inverted BRR1 origin of replication plasmid containing Kva1 recombitron with a donor in the ncRNA to target phzM locus in Pseudomonas aeruginosa PAO1 expressed by Pm promoter |
| 240705 | pRECMYC-Eco1 | pLAM12 vector backbone containing Eco1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240706 | pRECMYC-Eco2 | pLAM12 vector backbone containing Eco2 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240707 | pRECMYC-Ban1 | pLAM12 vector backbone containing Ban1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240708 | pRECMYC-Abe1 | pLAM12 vector backbone containing Abe1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240709 | pRECMYC-Kva1 | pLAM12 vector backbone containing Kva1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240710 | pRECMYC-Eas1 | pLAM12 vector backbone containing Eas1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240711 | pRECMYC-Csp1 | pLAM12 vector backbone containing Csp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240712 | pRECMYC-Tsp1 | pLAM12 vector backbone containing Tsp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240713 | pRECMYC-Ebu1 | pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |
| 240714 | pRECMYC-Hcl1 | pLAM12 vector backbone containing Hcl1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155 |