Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants.
Neubauer M, Vollen K, Zhao C, Ascencio-Ibanez JT, Hanley-Bowdoin L, Stepanova AN, Alonso JM
Plant Biotechnol J. 2025 Sep 21. doi: 10.1111/pbi.70320.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240715 | BCTVΔREP-pUPD2 | BCTV complementary sense ORFs with mutated Rep with GoldenBraid C1 grammar |
| 240716 | BCTV-VS WT-pUPD2 | WT BCTV virion sense ORFs prior to domestication for GoldenGate cloning with A1-B1 Goldenbraid grammar |
| 240717 | BCTVΔIR-pUPD2 | BCTV complementary sense ORFs lacking the IR. Has C1 Goldenbraid grammar. |
| 240718 | BCTVΔVS-pUPD2 | BCTV IR lacking virion sense ORFs. |
| 240719 | RT B2-B6-pUPD2 | GUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B2-B6 Goldenbraid grammar. |
| 240720 | RT B6-pUPD2 | GUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B6 Goldenbraid grammar. |
| 240721 | ZFN-pUPD2 | ZFN that targets the broken GUS:NPTII reporter with B2-B5 Goldenbraid grammar. |
| 240722 | BCTV-YPET-pDGB3alpha2 | BCTV vector for YPET expression. |
| 240723 | BCTVΔVS-YPET-pDGB3alpha1 | BCTV vector for YPET expression. Lacks the BCTV virion sense ORFs. |
| 240724 | BCTV-RT-pDGB3alpha2 | BCTV vector containing the GUS:NPTII repair template for HDR experiments. |
| 240725 | BCTVΔREP-YPET-pDGB3alpha1 | BCTV vector for YPET expression. Rep has been mutated. |
| 240726 | BCTVΔVSΔREP-YPET-pDGB3alpha1 | BCTV vector for YPET expression. Lacks the BCTV virion sense ORFs and Rep has been mutated. |
| 240727 | BCTVΔVSΔREP-RT-pDGB3alpha1 | BCTV vector containing the GUS:NPTII repair template for HDR experiments. Lacks the BCTV virion sense ORFs and Rep has been mutated. |
| 240728 | BCTVΔREP-RT-pDGB3alpha1 | BCTV vector containing the GUS:NPTII repair template for HDR experiments. Rep has been mutated. |
| 240729 | 35S-ZFN-pDGB3alpha1 | Encodes a ZFN that targets the broken GUS:NPTII reporter. |
| 240746 | BCTV-CS-pUPD2 | BCTV complementary sense ORFs with C1 Goldenbraid grammar |
| 240747 | BeYDV-RT-pUPD2 | BeYDV viral vector containing a repair template. |
| 240748 | BCTV-VS domesticated-pUPD2 | Domesticated BCTV virion sense ORFs with A1-B1 Goldenbraid grammar |