Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli.

Sepulchro AGV, Kozlowski HCJ, Norholm MHH
Commun Biol. 2025 Jun 17;8(1):933. doi: 10.1038/s42003-025-08361-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
240750	SAGA001	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240751	SAGA002	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240752	SAGA003	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240753	SAGA004	Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
240754	SAGA005	Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
240755	SAGA006	Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
240756	SAGA007	Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
240757	SAGA008	Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
240758	SAGA009	Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
240761	SAGA012	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240762	SAGA013	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240763	SAGA014	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240764	SAGA015	Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
240767	SAGA018	Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
240768	SAGA019	Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
240770	SAGA021	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240771	SAGA022	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240772	SAGA023	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240779	SAGA030	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240780	SAGA031	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240781	SAGA032	Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
240788	SAGA039	Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
240789	SAGA040	Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
240791	SAGA042	Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.
240792	SAGA043	Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.
240794	SAGA045	Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.
240795	SAGA046	Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.