A plasmid module for PCR-based gene modification for the accurate measurement of vacuolar delivery of specific proteins in yeast Saccharomyces cerevisiae.
Valdbjorn Kanne J, Reggiori F
Autophagy Rep. 2025 May 31;4(1):2511724. doi: 10.1080/27694127.2025.2511724. eCollection 2025.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240971 | pFA6a-Pho8∆60-His3MX6 | To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker |
| 240974 | pFA6a-Pho8∆60-BleMX6 | To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; BleMX6 selection marker |