Light-activated tetanus neurotoxin for conditional proteolysis and inducible synaptic inhibition in vivo
Roh H, Kim D, Kim B, Jeon Y, Kim Y, Jacko M, Xu F, Lin C, Won Um J, Ting AY
bioRxiv 2025.01.27.635161; doi: 10.1101/2025.01.27.635161
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 240975 | pAct1-LexA-VP16-VAMP2 + LexO-YFP | Expresses membrane-anchored transcription factor (LexA-VP16) in yeast, along with YFP reporter under LexO promoter |
| 240976 | pyGAIP-LATeNT-mCherry | Expresses LATeNT-mCherry in yeast cytosol |
| 240977 | pcDNA3-TeNT | Expresses TeNT in the mammalian cytosol |
| 240978 | pcDNA3-LATeNT | Expresses LATeNT in the mammalian cytosol |
| 240979 | pcDNA3-LATeNT(slow) | Expresses LATeNT* (slow reversing) in the mammalian cytosol |
| 240980 | pcDNA3-NanoLuc-LATeNT | Expresses NanoLuc-LATeNT in the mammalian cytosol |
| 240981 | pcDNA3-Ca-LATeNT | Expresses Ca-LATeNT in the mammalian cytosol |
| 240982 | pAAV-CCR6-NanoLuc | Expresses CCR6-NanoLuc in mammalian cells; used with LATeNT*-Arrestin |
| 240983 | pAAV-KORD-NanoLuc | Expresses KORD-NanoLuc in mammalian cells; used with LATeNT*-Arrestin |
| 240984 | pcDNA3-LATeNT(slow)-Arrestin | Expresses LATeNT*-Arrestin in mammalian cells; used with CCR6-NanoLuc or KORD-NanoLuc |
| 240985 | pcDNA3-Gal4-LATeNT(slow) | Expresses Gal4-LATeNT* cargo in mammalian cells; used with UAS-mCherry reporter |
| 240986 | pcDNA3-RELEASE-LATeNT | Expresses RELEASE-LATeNT cargo in mammalian cells; used with cytsolic LATeNT |
| 240987 | pcDNA3-LABoNT A | Expresses LABoNT/A in the mammalian cytosol |
| 240988 | pTRE3G-LATeNT | Dox-inducible expression of LATeNT in the mammalian cytosol; lentiviral vector |
| 240989 | pAAV-CAG-mCherry-LATeNT | Expresses mCherry-LATeNT in mammalian cells; for AAV transduction of neurons |
| 240990 | pAAV-hSyn-mCherry-TeNT | Expresses mCherry-TeNT in mammalian cells; for AAV transduction of neurons |
| 240991 | pAAV-EF1a-fDIO-mCherry-LATeNT | Flp-dependent expression of LATeNT |
| 240992 | pAAV-hSyn-DIO-mCherry-LATeNT | Cre-dependent expression of LATeNT |
| 240993 | pAAV-CaMKII-LATeNT | Expresses LATeNT in mammalian cells; CaMKII promoter |
| 240994 | pCAG-LATeNT | Expresses LATeNT in mammalian cells; CAG promoter |
| 240995 | pAAV-CAG-NanoLuc-LATeNT(slow) | Expresses NanoLuc-LATeNT* in neurons; for AAV transduction |