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Encryption and steganography of synthetic gene circuits.

Purcell O, Wang J, Siuti P, Lu TK
Nat Commun. 2018 Nov 22;9(1):4942. doi: 10.1038/s41467-018-07144-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85936pOP437Encrypted AND gate
85937pOP440Encrypted AND gate, decryption step 1.
85938pOP447Encrypted AND gate, decryption step 2.
85939pOP458Decrypted AND gate.
85940pOP512Bi-stable switch. From Collins lab but the origin substituted for a P15A origin.
85941pOP549Bi-stable switch, similar to pOP512 but with a lambdaCI OR_1 operator in ptrc-2 promoter driving tetR.
85942pOP523Encodes araC and LambdaCI. To be used with pOP549. Encrypts pOP549.
85943pOP576Temperature sensitive plasmid based on pDK46. Expresses dCas9 from proD. Serves as a control for pOP587 - pOP594
85944pOP587pOP576 with gRNA key
85945pOP588pOP576 with gRNA key
85946pOP589pOP576 with gRNA key
85947pOP590pOP576 with gRNA key
85948pOP591pOP576 with gRNA key
85949pOP592pOP576 with gRNA key
85950pOP593pOP576 with gRNA key
85951pOP594pOP576 with gRNA key

Antibodies from Article