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Targeted genetic screening in bacteria with a Cas12k-guided transposase.

Chen W, Ren ZH, Tang N, Chai G, Zhang H, Zhang Y, Ma J, Wu Z, Shen X, Huang X, Luo GZ, Ji Q
Cell Rep. 2021 Aug 31;36(9):109635. doi: 10.1016/j.celrep.2021.109635. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
171525pGTPAPlasmid contains the Cas12k gRNA and transposon donor cassette
171526pGTPA-eGFPPlasmid contains the Cas12k gRNA and transposon donor cassette assembled with eGFP
171527pGTPA-GmPlasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant gene
171528pGTPA-lbPlasmid contains the Cas12k gRNA for mutant library construction

Antibodies from Article