Targeted genetic screening in bacteria with a Cas12k-guided transposase.
Chen W, Ren ZH, Tang N, Chai G, Zhang H, Zhang Y, Ma J, Wu Z, Shen X, Huang X, Luo GZ, Ji Q
Cell Rep. 2021 Aug 31;36(9):109635. doi: 10.1016/j.celrep.2021.109635.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
171525 | pGTPA | Plasmid contains the Cas12k gRNA and transposon donor cassette |
171526 | pGTPA-eGFP | Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with eGFP |
171527 | pGTPA-Gm | Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant gene |
171528 | pGTPA-lb | Plasmid contains the Cas12k gRNA for mutant library construction |