All Probes Plasmids (APPs) for multicolor and long-term tracking of single-mRNA translation dynamics.
Galindo G, Fixen GM, Heredia A, Morisaki T, Stasevich TJ
Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi: 10.1091/mbc.E25-02-0091.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 241135 | pCMV-Frankenbody-HA-mScarlet3-IRESv4-HaloTag-tdMCP | Expresses Anti-HA frankenbody-mScarlet3 and HaloTag-tdMCP to track mature and nascent HA-tagged proteins and MS2-tagged mRNA |
| 241137 | pCMV-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP | Expresses Anti-GCN4 scFv-sfGFP and HaloTag-tdMCP to track mature and nascent SunTag-tagged proteins and MS2 tagged mRNA |
| 241139 | pCMV-Frankenbody-HA-mScarlet3-tdP2A-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP | Expresses Anti-HA frankenbody-mScarlet3, anti-GCN4 scfv-sfGFP, and HaloTag-tdMCP to track mature and nascent HA and SunTag-tagged proteins and MS2 tagged mRNA |
| 241140 | pCMV-Frankenbody-HA-mScarlet3-tdP2A-Frankenbody-FLAG-mEGFP-IRESv4-HaloTag-tdMCP | Expresses Anti-FLAG frankenbody-mScarlet3, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent HA and FLAG-tagged proteins and MS2-tagged mRNA |
| 241141 | pCMV-scFv-GCN4-sfGFP-tdP2A-Frankenbody-FLAG-mScarlet3-IRESv4-HaloTag-tdMCP | Expresses Anti-GCN4 scFv-sfGFP, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent SunTag and FLAG-tagged proteins and MS2-tagged mRNA |
| 241144 | pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX | Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane |
| 241145 | pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-CAAX | Expresses Anti-HA frankenbody-StayGold and HaloTag-tdMCP-CAAX to track mature and nascent SunTag-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane |