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Unusually Broad-spectrum small-molecule sensing using a single protein scaffold.

Tian H, Beltran J, George W, Lenert-Mondou C, Seder N, Davis ZI, Swift SD, Girke T, Whitehead TA, Wheeldon I, Cutler SR
bioRxiv [Preprint]. 2025 May 15:2025.05.15.654352. doi: 10.1101/2025.05.15.654352. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
241285pBD_PYR1_156Backbone for PYR1 sensor library assembly from oligopools; domesticated for golden gate cloning
241288pBD-PYR1-TSM_libraryLibrary of pBD-PYR1 triple site mutants for yeast-based sensor screens
241289pBD-PYR1-DSM_Hao_libraryLibrary of pBD-PYR1 double site mutants for yeast-based screens; redesigned to remove constitutive mutants and wild type rceptors

Antibodies from Article