A PIP2-stabilized syntaxin-1a structure mapped with transition metal ion FRET and unnatural fluorescent amino acids at the plasma membrane.
Obashi K, Strub MP, Taraska JW
Structure. 2025 Nov 24:S0969-2126(25)00388-0. doi: 10.1016/j.str.2025.10.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 241290 | pANAP-P2A-eRF(E55D) | Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor |
| 241291 | Syntaxin1A(E49TAG)-mCherry | Mammalian expression of syntaxin-1(E49TAG) fused to mCherry for genetic code expansion via Amber codon supression |
| 241292 | Syntaxin1A(E49TAG,G42H,K46H)-mCherry | Mammalian expression of syntaxin-1(E49TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression |
| 241293 | Syntaxin1A(E53TAG)-mCherry | Mammalian expression of syntaxin-1(E53TAG) fused to mCherry for genetic code expansion via Amber codon supression |
| 241294 | Syntaxin1A(E53TAG,G42H,K46H)-mCherry | Mammalian expression of syntaxin-1(E53TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression |
| 241295 | Syntaxin1A(E206TAG)-mCherry | Mammalian expression of syntaxin-1(E206TAG) fused to mCherry for genetic code expansion via Amber codon supression |
| 241296 | Syntaxin1A(R210H,E206TAG)-mCherry | Mammalian expression of syntaxin-1(E206TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression |
| 241297 | Syntaxin1A(K84TAG)-mCherry | Mammalian expression of syntaxin-1(K84TAG) fused to mCherry for genetic code expansion via Amber codon supression |
| 241298 | Syntaxin1A(R210H,K84TAG)-mCherry | Mammalian expression of syntaxin-1(K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression |
| 241299 | Syntaxin1A(I233A,K84TAG)-mCherry | Mammalian expression of syntaxin-1(I233A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression |
| 241300 | Syntaxin1A(I233A,R210H,K84TAG)-mCherry | Mammalian expression of syntaxin-1(I233A,K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression |
| 241301 | Syntaxin1A(K264A,K265A,K84TAG)-mCherry | Mammalian expression of syntaxin-1(K264A,K265A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression |
| 241302 | Syntaxin1A(K264A,K265A,R210H,K84TAG)-mCherry | Mammalian expression of syntaxin-1(K264A,K265A,K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression |
| 241303 | mVenus-synaptojanin1(498-901)-CAAX | Mammalian expression of truncated form of synaptojanin-1 with CAAX motif fused to mVenus |
| 241304 | mVenus-synaptojanin1(D730A,498-901)-CAAX | Mammalian expression of truncated form of 5-phosphatase-dead synaptojanin-1 with CAAX motif fused to mVenus |
| 241305 | Lyn-FRB_star-P2A-mVenus-FKBP-5ptase | Mammalian expression of Lyn-FRB_star and mVenus-FKBP-5ptase of INPP5E |
| 241306 | Lyn-FRB_star-P2A-mVenus-FKBP-5ptase(D343A) | Mammalian expression of Lyn-FRB_star and mVenus-FKBP-5ptase(phosphatase-dead mutant) of INPP5E |